Part:BBa_K801038:Design

PhyB-linker-Gal4bd

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 487
  Illegal BamHI site found at 569
  Illegal XhoI site found at 520
  Illegal XhoI site found at 539
  Illegal XhoI site found at 2674
  Illegal XhoI site found at 3008
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2059
  Illegal BsaI site found at 2465
  Illegal BsaI site found at 2927
  Illegal SapI site found at 736
  

Composite part of BBa_K801031, Bba_K365005 and BBa_K801032

Keywords: light switchable, Gal4, PIF3, phytochrome B

Abbreviations:

Design Notes

Related BioBrick:

• Related BioBricks:BBa_K801031: Phytochrome B, BBa_K365005: 20 aa linker RFC-25 , BBa_K801032: GAL4 DNA binding domain

Cloning details:

• Designed in RFC25

Quality control measures:

Backbone:

• Backbone name: pSB1C3

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

References

Literature references:

Database references: